/*! This file is auto-generated */ .wp-block-button__link{color:#fff;background-color:#32373c;border-radius:9999px;box-shadow:none;text-decoration:none;padding:calc(.667em + 2px) calc(1.333em + 2px);font-size:1.125em}.wp-block-file__button{background:#32373c;color:#fff;text-decoration:none} Q8 P 18-8: What is an absorption spec... [FREE SOLUTION] | ÷ÈÓ°Ö±²¥

÷ÈÓ°Ö±²¥

18-8: What is an absorption spectrum?

Short Answer

Expert verified

The absorption spectrum of a chemical is the part of the incident radiation that the substance hasabsorbedin a certain frequency range. The absorption spectrum is primarily determined by theatomic and molecularcomposition of the chemical. It is a graph of absorbance versus wavelength.

Step by step solution

01

Principle of the Absorption spectrum

Atomic Absorption spectroscopy (AAS) is based upon the principle that free atoms in the ground state can absorb light of a certain wavelength. Absorption for each element is specific, no other elements absorb this wavelength

02

Example of Absorption spectrum

The pattern of absorption lines in a spectrum is diagnostic of the types of atoms and molecules present, for example, in the surface layers of a star or the atmosphere of a planet. Absorption lines are seen in the spectra of the Sun and other stars.

03

 Step 3: Uses of absorption spectrum

Agriculture– analyzing soil and plants for minerals necessary for growth

Environmental Study– determination of heavy metals in water, soil, and air

Food Industry– quality assurance and testing for contamination

Forensic’s – substance identification

Mining– testing the concentration of valuable substances in potential mining areas

Nuclear Energy– monitoring potentially hazardous elements in water and waste output

Petrochemical– analyzing products for metals and other substances that can have adverse affects such as oil and gas

Pharmaceutical– many applications from quality control to detecting impurities in drugs

Unlock Step-by-Step Solutions & Ace Your Exams!

  • Full Textbook Solutions

    Get detailed explanations and key concepts

  • Unlimited Al creation

    Al flashcards, explanations, exams and more...

  • Ads-free access

    To over 500 millions flashcards

  • Money-back guarantee

    We refund you if you fail your exam.

Over 30 million students worldwide already upgrade their learning with ÷ÈÓ°Ö±²¥!

One App. One Place for Learning.

All the tools & learning materials you need for study success - in one app.

Get started for free

Most popular questions from this chapter

18-26. A 2.00mL solution of apotransferrin was titrated as illustrated in Figure 18-11. It required 163L of 1.43 mM ferric nitrilotriacetate to reach the end point.

(a) Why does the slope of the absorbance versus volume graph change abruptly at the equivalence point?

(b) How many moles of Fe(III) (= ferric nitrilotriacetate) were required to reach the end point?

(c) Each apotransferrin molecule binds two ferric ions. Find the molar concentration of apotransferrin in the 2.00mL solution.

What is the difference between fluorescence and phosphorescence?

Vapor at a pressure of 30.3μ³Ùbar from the solid compound pyrazine had a transmittance of 24.4% at a wavelength of 266nm in

a 3.00 - cm cell at 298K

Pyrazine

(a) Convert transmittance to absorbance.

(b) Convert pressure to concentration (mol/L) with the ideal gas law (Problem 1-18).

(c) Find the molar absorptivity of gaseous pyrazine at 266nm.

18-29. Biotin-streptavidin fluorescence titration. Biotin is a cofactor in enzymatic carboxylation reactions. Biotin activatesCO2for biosynthetic reactions.


Streptavidin is a protein isolated from the bacterium Streptomyces avidinii that binds biotin with a formation constant of ~1014M-1. The biotin-streptavidin complex is widely used in biotechnology because the noncovalent complex is stable in the presence of detergents, protein denaturants, and organic solvents, and at extremes of pH and temperature.

The stoichiometry of the biotin-streptavidin complex was measured by a fluorescence titration. Fluorescein (page 453 ) covalently attached to biotin via the biotin carboxyl group fluoresces at 520 nm when irradiated at 493 nm. When biotin-fluorescein (BF) binds to streptavidin (SA), fluorescence decreases. The table gives emission intensity for addition of BF to SA and also for addition of SA to BF. Data are already corrected for dilution.

(a) Make a graph of fluorescence versus mole ratio for each titration and state the stoichiometry of binding of biotin to streptavidin.

(b) Explain the shape of each titration curve.

0.10mMKMnO4has an absorbance maximum of 0.26 at 525 nm in a

1.000-cm cell. Find the molarabsorptiveand the concentration of a solution whose

absorbance is 0.52 at 525 nm in the same cell.

See all solutions

Recommended explanations on Chemistry Textbooks

View all explanations

What do you think about this solution?

We value your feedback to improve our textbook solutions.

Study anywhere. Anytime. Across all devices.