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In tomatoes, red fruit is dominant over yellow, twoloculed fruit is dominant over many-loculed fruit, and tall vine is dominant over dwarf. A breeder has two pure lines: (1) red, two-loculed, dwarf and (2) yellow, many-loculed, tall. From these two lines, he wants to produce a new pure line for trade that is yellow, twoloculed, and tall. How exactly should he go about doing so? Show not only which crosses to make, but also how many progeny should be sampled in each case.

Short Answer

Expert verified
Cross the two lines, select F2 plants based on phenotype, sample at least 128 to increase success chance.

Step by step solution

01

Understanding Dominance

Red fruit is dominant over yellow, twoloculed is dominant over many-loculed, and tall vine is dominant over dwarf. We represent this by assigning dominant alleles: R (red), T (twoloculed), and V (tall). The recessive alleles are represented by r (yellow), t (many-loculed), and v (dwarf).
02

Identifying Initial Genotypes

We have two pure lines: 1. Red, two-loculed, dwarf - Genotype: RRTTvv 2. Yellow, many-loculed, tall - Genotype: rrttVV. Our goal is to produce a pure line that is yellow (rr), twoloculed (TT), and tall (VV).
03

Creating F1 Generation

Cross the two initial pure lines: RRTTvv with rrttVV. The offspring (F1 generation) will be heterozygous with genotype RrTtVv. These will all be phenotypically red, twoloculed, and tall due to dominance.
04

Generating F2 Generation

From the F1 generation, perform a self-cross (RrTtVv x RrTtVv) to produce the F2 generation. This cross will allow for the segregation and recombination of alleles, creating varied genotypes.
05

Selecting Desired Phenotype

In the F2 generation, we want plants that express the yellow, twoloculed, tall phenotype. The genotype we are aiming for is rrTTVV. To find a recessive phenotype (yellow), sample a large number of plants.
06

Calculating Probability

The probability for each trait to be homozygous recessive: for yellow (rr), the probability is 1/4; for twoloculed (TT), the probability is 1/4; for tall (VV), the probability is 3/4. Multiply these probabilities: \[\frac{1}{4} \times \frac{1}{4} \times \frac{3}{4} = \frac{3}{64}\]Thus, there is a 3/64 chance of getting the desired genotype per plant.
07

Estimating Sample Size

To ensure a high chance of getting at least one plant with the desired genotype (rrTTVV), a large number of offspring should be sampled. For example, sampling 128 plants gives more than a 95% chance (1 - (1 - 3/64)^128) of obtaining at least one rrTTVV plant.

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Key Concepts

These are the key concepts you need to understand to accurately answer the question.

Mendelian Inheritance
Mendelian inheritance refers to the patterns of inheritance first explained by Gregor Mendel in the 19th century. It's the foundational framework for understanding how traits are passed from parents to offspring. Mendelian inheritance involves genes, which are units of heredity located on chromosomes. Genes come in different forms called alleles, which can be dominant or recessive.

In this case study with tomatoes, we see Mendel's principles in action: we have dominant alleles (R, T, V) for red, twoloculed, and tall traits respectively, and recessive alleles (r, t, v) for yellow, many-loculed, and dwarf traits. When two organisms reproduce, their alleles interact to determine the traits of their offspring. Understanding these interactions allows breeders to predict plant outcomes efficiently.

  • Dominant alleles mask the presence of recessive alleles.
  • Mendel's Laws include the Law of Segregation and the Law of Independent Assortment, both of which apply in our tomato genetic crosses.
By following these principles, breeders can strategically cross plants to develop the desired genotypes and phenotypes.
Dominant and Recessive Traits
Dominant and recessive traits are essential concepts in genetics. Dominant alleles are those that overpower recessive alleles in defining a trait. For a trait governed by a dominant allele to be expressed, only one copy of the dominant allele is necessary.

In our exercise, we have three dominant traits: red fruit, twoloculed fruit, and tall vines. Correspondingly, yellow fruit, many-loculed fruit, and dwarf vines are recessive traits. Here's how they work:

  • A tomato plant needs at least one R allele to exhibit red fruit.
  • Two T alleles are needed for a plant to be twoloculed, though the exercise aims for the contrary in recessive trait exploration.
  • Tall vine requires at least one V allele.
With these dominant traits, even when a plant carries one dominant and one recessive allele, the dominant trait will be visible. This knowledge helps breeders plan cross-breeding strategies to achieve specific plant outcomes.
Phenotype and Genotype
Phenotype and genotype are two sides of the same genetic coin. A phenotype refers to observable traits or characteristics, such as the color of the fruit or the height of the plant. A genotype, on the other hand, describes the genetic makeup that dictates these traits.

The original phenotypes in this exercise are red, two-loculed, and dwarf tomatoes, and yellow, many-loculed, and tall tomatoes. The genotypes RRTTvv and rrttVV support these phenotypic expressions. Understanding the genotype behind each phenotype is vital to predicting future generations.

In the exercise, creating the desired yellow, twoloculed, and tall phenotype (rrTTVV) involves careful selection of plant genotypes. By manipulating which alleles are present in offspring through controlled breeding, breeders can pinpoint a desired phenotype, which aligns with their market objectives or research goals.
Probability Calculation in Genetics
Probability calculation is a crucial aspect of genetic crosses. It allows breeders to quantify the chances of certain genotypes appearing in offspring. In tomato breeding, we're looking at probabilities to guide us toward the desired yellow, twoloculed, and tall phenotype.

Several genetic principles determine these probabilities:

  • The chance of getting a homozygous recessive trait (like rr for yellow) from a heterozygous pair (Rr x Rr) is \(\frac{1}{4}\).
  • The likelihood of inheriting a dominant twoloculed trait (TT) from an intermediate generation is also \(\frac{1}{4}\), assuming heterozygosity.
  • For a dominant tall trait (VV), presence in any generation stands at a probability of \(\frac{3}{4}\).
Given these probabilities, the overall chance of a plant having the genotype rrTTVV is calculated as \(\frac{3}{64}\). This figure helps in planning how many plants need to be sampled to ensure a high probability of obtaining at least one plant with the ideal combination, using statistical principles.

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Most popular questions from this chapter

A man is brachydactylous (very short fingers; rare autosomal dominant \()\) and his wife is not. Both can taste the chemical phenylthiocarbamide (autosomal dominant; common allele), but their mothers could not. a. Give the genotypes of the couple. If the genes assort independently and the couple has four children, what is the probability of b. all of them being brachydactylous? c. none being brachydactylous? d. all of them being tasters? e. all of them being nontasters? f. all of them being brachydactylous tasters? g. none being brachydactylous tasters? h. at least one being a brachydactylous taster?

We have dealt mainly with only two genes, but the same principles hold for more than two genes. Consider the following cross: \(A / a ; B / b ; C / c ; D / d ; E / e \times a / a ; B / b ; c / c ; D / d ; e / e\) a. What proportion of progeny will phenotypically resemble (1) the first parent, (2) the second parent, (3) either parent, and (4) neither parent? b. What proportion of progeny will be genotypically the same as (1) the first parent, (2) the second parent, (3) either parent, and (4) neither parent?? Assume independent assortment.

One form of male sterility in corn is maternally transmitted. Plants of a male-sterile line crossed with normal pollen give male-sterile plants. In addition, some lines of corn are known to carry a dominant nuclear restorer allele \((R f)\) that restores pollen fertility in male-sterile lines. a. Research shows that the introduction of restorer alleles into male-sterile lines does not alter or affect the maintenance of the cytoplasmic factors for male sterility. What kind of research results would lead to such a conclusion? b. A male-sterile plant is crossed with pollen from a plant homozygous for \(R f\). What is the genotype of the \(\mathrm{F}_{1} ?\) The phenotype? c. The \(F_{1}\) plants from part \(b\) are used as females in a testcross with pollen from a normal plant \((r f / r f)\). What are the results of this testcross? Give genotypes and phenotypes, and designate the kind of cytoplasm. d. The restorer allele already described can be called \(R f-1 .\) Another dominant restorer, \(R f \cdot 2,\) has been found. \(R f \cdot 1\) and \(R f-2\) are located on different chromosomes. Either or both of the restorer alleles will give pollen fertility. With the use of a male-sterile plant as a tester, what will be the result of a cross in which the male parent is (i) heterozygous at both restorer loci? (ii) homozygous dominant at one restorer locus and homozygous recessive at the other? (iii) heterozygous at one restorer locus and homozygous recessive at the other? (iv) heterozygous at one restorer locus and homozygous dominant at the other?

Assume independent assortment and start with a plant that is dihybrid \(A / a ; B / b\) a. What phenotypic ratio is produced from selfing it? b. What genotypic ratio is produced from selfing it? c. What phenotypic ratio is produced from testcrossing it? d. What genotypic ratio is produced from testcrossing it?

In dogs, dark coat color is dominant over albino and short hair is dominant over long hair. Assume that these effects are caused by two independently assorting genes, and write the genotypes of the parents in each of the crosses shown here, in which \(\mathrm{D}\) and \(\mathrm{A}\) stand for the dark and albino phenotypes, respectively, and \(\mathrm{S}\) and \(\mathrm{L}\) stand for the short-hair and long-hair phenotypes.

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